High performance liquid chromatography of long-chain acylcarnitine and phospholipids in fatty acid turnover studies

High performance liquid chromatography of long-chain acylcarnitine and phospholipids in fatty acid turnover studies

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In this paper we describe a rapid, isocratic high performance liquid chromatography (HPLC) method for the study of radioactive fatty acid incorporation into complex lipids of human erythrocytes, which Casting - Cast Padding allows the simultaneous separation of the major phospholipid classes and long-chain acylcarnitines.The lipid extract of erythrocytes pulsed with radioactive fatty acids was injected into an HPLC system equipped with a silica column.The individual components eluted were monitored by ultraviolet absorption and radioactive emission.

With respect to Dishes the UV profile, the radioactive profile showed an additional peak between phosphatidyl-choline and phosphatidylethanolamine, which was identified as long-chain acylcarnitine by different experimental approaches.The radioactivity recovered in the long-chain acylcarnitines contains essential information enabling definition of acyl trafficking in red cells.